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Coding

Part:BBa_K1590000:Design

Designed by: Manuel Blank   Group: iGEM15_Dundee   (2015-09-08)


Human Haemoglobin A


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 342
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Choosing the correct codon-bias, remove 'illegal' restriction sites, ensure that (stop)codons are in frame, addition of standard prefix and suffix.

Primers for cloning Haemoglobin A into overexpression vector pQE80-L. 

   Forward: 5' GCGC GGATCC GTTCTGTCTCCGGCGGAC 3'
   Reverse: 5' GCGC GGTACC TTACGGTATTTAGAGGT 3'

Primers for cloning Haemoglobin A into vector for bacterial 2-hybrid system, pT25. 

   Forward: 5' GCGC GGATCC AGTTCTGTCTCCGGCGGAC 3'
   Reverse: 5' GCGC GGTACC TTACGGGGTGAACTCCGCCGG 3'

Part was synthesised with appropriate prefix/suffix sequences, hence no separate primers were required for cloning it into pSB1C3.

Source

In silico optimised version for E. coli K12 strains. Sequence ordered through IDT.

References